Leica TCS SP8 SMD, User Manual

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No additional pulsed laser 470 or 640 for MP systems
Systems equipped with an MP laser cannot be complemented with a
pulsed 440, 470, or 640-nm laser.
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FLIM Data Acquisition
16.2.2.4 Using White Light Lasers
1. To activate the laser, follow the instructions in Chapter 15.1 . In general we recommend
using the filters available in the fluorifier disc.
2. If the white light laser is used as an excitation source for FLIM measurements, always
use only one wavelength per FLIM measurement.
16.2.3 Adjusting the Fluorifier Disc
With the Show Fluorifier Settings button ( Figure 125 ), you can call up the Fluorifier Disc
Settings dialog to select a barrier filter or set a polarization angle.
16.2.3.1 Setting for SP FLIM
SMD systems equipped with SP FLIM detectors have a fluorifier disc with barrier filters for
the excitation light. These filters prevent reflected excitation light from hitting the SP
module.
Alternative selection of UV and MP FLIM excitation
Some systems feature both a pulsed diode laser (405 nm) and an MP
laser. Only one of these two lasers can be used for FLIM at a time. Make
sure that the correct synchronization cable is connected to the TCSPC
unit PicoHarp 300 at channel 0. When changing between MP and 405 nm
excitation for FLIM, you must also change the synchronization cable
connected to the PicoHarp.
Alternative selection of excitation from a white light laser, pulsed
diode, or MP FLIM
Some systems feature not only a white light laser, but also a pulsed laser
and/or an MP laser. Only one of these three laser options can be used for
FLIM at a time. Make sure that the correct synchronization cable is
connected to the TCSPC unit PicoHarp 300 at channel 0. When changing
between MP, white light laser, or diode laser for FLIM, you must also
change the synchronization cable connected to the PicoHarp.