Leica TCS SP8 SMD, User Manual

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F(L)CS Data Acquisition
Figure 170: Settings for using pulsed VIS lasers as excitation source
4. Check whether the correct beam splitter ( MFP ) is selected ( Table 18, page 123 ).
5. You can use the pulsed VIS lasers together with the pulsed UV laser (405 nm). The MFP
setting is not influenced by UV. It is defined by the selection of the pulsed VIS lasers.
18.2.2.4 Using a Pulsed UV Laser
1. Open the shutter for pulsed lasers.
2. Activate the 405-nm laser by moving the corresponding slider.
3. If no other pulsed laser is used, you can set the multifunction port (
MFP
) to
Substrate
.
Controlling laser intensity of the pulsed laser
For pulsed lasers, the slider does not affect the laser intensity. Laser
intensity needs to be controlled at the desired laser.
Combination pulsed and continuous wave VIS excitation
Do not use any pulsed VIS excitation together with continuous wave VIS
excitation for continuous wave laser lines close to the wavelengths of
the pulsed laser (such as the combination 470 nm 488 nm
continuous wave), as no optimum MFP setting exists for such a
combination.