Leica TCS SP8 SMD, User Manual

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105
LAS AF
The displayed tabs depend on the selected step. In the TCS SP8's basic operating mode, the
following tabs are assigned to the steps:
• Acquire step:
Experiments
tab: Directory tree for opened files
Acquisition tab: Hardware settings for the current experiment and parameter settings
for image acquisition
• Process step:
Experiments
tab: Directory tree for opened files
Process Tools tab: Overview of the functions available in this step
•
Quantify
step:
Experiments tab: Directory tree for opened files
Tools
tab: Overview of the functions available in this step
Working area:
Depending on the step and tab selected, different dialogs are available in the working area:
•
Acquire
step:
Beam Path Settings: In this dialog, the beam path is shown schematically. You can select
and adjust the lasers and the system components along the beam path and the detectors
for image acquisition.
•
Process
step:
Appropriate processing options and a preview image appear in the working area based
on which function you have selected in the
Process Tools
tab.
• Quantify step:
The working area is divided into two tabs:
Graphs: Graphic representation of values that were measured in the entire image or in
regions of interest (ROI)
Statistics: Display of statistical values that were determined in the entire image or in
plotted regions of interest (ROI)
Image display
:
Acquired images can be displayed and regions of interest (ROI) and additional information
can be plotted here.
Image display settings :
This area contains buttons used to change the display options for acquired images (such as
color).
Image processing and image analysis options:
This area contains buttons that can be used to add information to the image, such as text,
arrows or dimensions. In addition, there is a button that enables regions of interest (ROI) to
be plotted in the image display.
Channel display:
Here, you can select how the acquired image is to be displayed. If you have excited your
specimen with multiple laser lines, you can use the buttons to select whether the different
channels are displayed individually, next to each other or in one image.
Image acquisition buttons
:
These buttons are available to you during the
Acquire
and
Quantify
steps. Here you can