Leica TCS SP8 SMD, User Manual

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Laser
10.4 Overview of usable lasers for FLIM
10.4.1 VIS/UV Lasers for TCS SP8 SMD
Laser type Wavelength
(nm) Maximum
light output at
laser output
(mW) Maximum
light output in
focal plane
(mW) Pulse duration
405 pulsed 405 3 @ 40 MHz 0.1 @ 40 MHz pulsed, >80 ps
405 dual-voltage 405 3 @ 40 MHz 0.1 @ 40 MHz continuous
wave (cw)
>80 ps
440 440 3 @ 40 MHz 0.1 @ 40 MHz pulsed, >80 ps
470 470 3 @ 40 MHz 0.3 @ 40 MHz pulsed, >80 ps
640 640 4.5 @ 40 MHz 0.5 @ 40 MHz pulsed, >80 ps
White light laser 470 – 670 < 500 < 50 Pulsed
The TCS SP8 SMD (for FLIM) features a combination of the lasers listed below:
10.4.2 Infrared Lasers for TCS SP8 SMD with MP Configuration
The TCS SP8 SMD with MP configuration (for FLIM) can contain a combination of all the
infrared lasers in
Chapter 10.4.1 and the following infrared lasers:
10.4.2.1 Picosecond laser
Laser type Wavelength
(nm) Maximum
light output at
laser output
(W) Maximum
light output in
focal plane
(W) Pulse duration
MaiTai HP ps 690 - 1040 < 3,5 < 1,9 pulsed, 1.0 - 1.5 ps
Chameleon Ultra II ps 680 - 1080 < 4 < 1,9 pulsed, 1.0 - 1.5 ps
10.4.2.2 Femtosecond laser
Laser type Wavelength
(nm) Maximum
light output at
laser output
(W) Maximum
light output in
focal plane
(W) Pulse duration
MaiTai fs 780 - 920 < 1,2 < 0,6 pulsed ~ 80 fs
MaiTai HP fs 690 - 1040 < 3,5 < 1,9 pulsed ~ 100 fs
MaiTai HP
Deep See 690 - 1040 < 3,5 < 1,9 pulsed ~ 100 fs
MaiTai eHP
Deep See 690 - 1040 < 3,5 < 1,9 pulsed ~ 70 fs
Chameleon Ultra I fs 690 - 1040 < 4 < 1,9 pulsed ~ 140 fs
Chameleon Ultra II fs 680 - 1080 < 4 < 1,9 pulsed ~ 140 fs