Leica TCS SP8 SMD, User Manual

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F(L)CS Data Acquisition
Figure 176: Starting the FCS test and applying the measuring point
5. To end illumination of the specimen, click the
Stop FCS Test
button in LAS AF. This will
also stop the online correlation display in SymPhoTime.
6. If you would like to reuse a tested FCS measuring point later for the 'correct' FCS
measurement, click Add Point ( Figure 176, item 2 ) in the Setup tab. This measuring point
is then written to a position list, which is used in the
Measurements
step in LAS AF. Do
not make any more changes to the zoom. Changing the zoom in the Setup FCS step would
render the position list invalid.
The instrument parameters set for the FCS measurements (laser lines and associated
intensity, pinhole, AOBS) are kept as separate FCS settings that are independent from the
image acquisition setting defined in the
Setup Imaging
step. The FCS settings defined in the
Setup FCS
step are automatically transferred to the
Measurements
step.
18.3.6 Count Rate Monitor
The count rate of each channel is displayed in the Setup tab. For FCS, the count rate should
be between 10,000 and 500,000 cps (counts per second).
If the two detection channels are reversed, change the allocation in SymPhoTime. The
allocation of the channels is preset and should not be changed.
• FCS Channel A: Check box
1
• FCS Channel B: Check box 2
Saving the data in Measurements
This curve is not saved. If you want to save your data, you have to work in
the
Measurements
step.