Leica TCS SP8 SMD, User Manual

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FLIM Data Acquisition
Figure 116: Dialog for AOBS configuration
2. Choose a suitable laser line and adjust the intensity to the desired level using the AOTF
slider.
3. If the system has a multifunction port ( MFP ), set it to Substrate .
16.1.2.2 Using MP Lasers
1. For MP scanning operations, select the desired wavelength in Beam Path Settings .
2. Choose a suitable neutral density filter for the attenuation using the Trans slider and/or
adjust the electro-optical modulator (EOM) using the Gain and Offset sliders.
3. If the system has a multifunction port, it should be set to SP680 or SP700 .
For more detailed information about switching on the MP lasers, refer to Chapter 15.1.2 .
16.1.2.3 Using Pulsed VIS Lasers
1. Close the shutter of the continuous wave VIS laser in Beam Path Settings and move all
sliders to the 0 position.
2. Open the shutter for pulsed lasers by activating the corresponding button ( Figure 117,
item 1 ).
3. Activate the desired laser by moving the corresponding slider upwards ( Figure 117,
item 2 ).
Prevent light incidence during MP FLIM measurement
During an MP FLIM measurement (particularly with HyD RLD), the light in
the room should be switched off and the shutter of the fluorescence lamp
should be closed. Furthermore, the iris diaphragm on the condenser
should be completely closed.
Controlling laser intensity of the pulsed laser
For pulsed lasers, the slider does not affect the laser intensity. Laser
intensity needs to be controlled at the desired laser.