Leica TCS SP8 SMD, User Manual

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General Safety Notes
• Never expose your eyes or skin to direct or indirect laser radiation. The radiation can
cause irreparable eye and skin injuries.
• During the scanning operation, the laser radiation is accessible in the microscope's
specimen area without obstruction after coming out of the objective. Always maintain a
nominal ocular hazard distance of at least 20 cm (8") between your eyes and the opening
of the objective.
Figure 1: Specimen area of upright and inverted microscope
• Make sure that the fiber optic cables are not damaged. The system may not be turned on
with damaged fiber optic cables, as laser radiation can escape and lead to irreparable
eye and skin injuries.
• It is not necessary to wear protective eyewear when using VIS and UV systems. When
used as intended and safety notes have been followed, laser radiation is kept within the
limit value that eliminates the chance for eye injuries.
• Do not use an S70 microscope condenser. The large working distance and the low
numerical aperture of the S70 microscope condenser could result in a hazard from laser
radiation.
• Only use S1 and S28 Leica microscope condensers.
• Do not look into the eyepieces during the scanning operation.
• Never change samples during a scanning operation.
• Never change objectives, filter cubes, beam splitters, condensers or other components
during a scanning operation.
• Do not look into the eyepieces when switching the beam path in the microscope.
• Do not introduce any reflective objects or mirrors into the laser beam path or into the
specimen area.
• If there is no lamp housing or mirror housing connected to the microscope, attach the
cover to the replacement flange.
• All unoccupied positions in the objective nosepiece must be closed using the supplied
caps.
• For MP systems, dry objectives (air objectives) may not be used with a numerical
aperture (NA) larger than 0.85. This does not apply to immersion objectives (oil, water).