Leica TCS SP8 SMD, User Manual

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LAS AF
Figure 87: Overview of the FLIM Wizard steps
• "
Setup Corr-ring
" step Some immersion objectives have a correction ring to optimally
correct for differences in the refractive index between immersion and specimen and
temperature effects. An optimum adjustment of the correction ring correlates with the
best possible optical resolution of the system. In the
Setup Corr-ring step, the beam path
is preconfigured for a fast and efficient adjustment of the correction ring.
• Setup Imaging workstep: Here, you can establish and optimize the conditions for the
acquisition of a reference image. In the
Setup Imaging
step, the FLIM data acquisition
cannot be started.
• Step
Setup FLIM
: Here, you can define and optimize instrument parameters for the FLIM
measurement. The FLIM measurement instrument parameters (such as selection of the
laser line and intensity, image size, pinhole size, etc.) are managed independently of the
parameters for image acquisition in the
Setup Imaging step. The optimal laser intensity
can be determined with a FLIM test measurement (selecting the
FLIM Test
button).
• Step Measurements : Here, you can define the FLIM experiment (exposure time of the
individual FLIM images, definition of FLIM volume stacks, FLIM time series or, for internal
SP FLIM, FLIM lambda stacks).