Leica TCS SP8 SMD, User Manual

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219
Changing the Filter Cube
23 Changing the Filter Cube
SMD filter cubes for the following dye combinations are available for the Leica SMD system:
Table 22: List of available SMD filter cubes
WARNING Risk of permanent eye damage from laser radiation
Never change filter cubes during the scanning operation because laser
radiation can escape uncontrolled from the specimen area.
Dye
combination Maximum
number of
external
detection
channels
Possible
excitation lines
Beam
splitter
Bandpass
I
Bandpass II
neutral 2 50/50 % - -
Polarization 2 Polarization - -
GFP wide / RFP 2 458, 470, 488, 561,
594
LP 560 500 – 550 607 – 683
Alexa 488 /
Alexa 633 2 458, 470, 488, 561,
594, 633
LP 620 500 – 550 647 – 703
CFP / YFP 2 405, 458, 514 LP 505 467 – 499 535 – 585
CFP/RFP 2 405, 458, 561, 594 LP 560 470 – 550 607 – 683
GFP narrow /
RFP 2 458, 470, 488, 543,
561, 594
LP 560 500 – 530 607 – 683
GFP / RFP 2 458, 470, 488, 561,
594
LP 560 500 – 550 607 – 683
FITC/TRITC 2 458, 470, 488, 543 LP 560 500 – 550 565 – 605
GFP/HcRed 2 458, 470, 488, 561 LP 560 500 – 550 581 – 654
Alexa 405/ cy2 2 405, 470, 488 LP 470 418 – 458 500-550
DAPI, Atto 647 2 405, 514, 543, 561,
594, 633, 640
LP 620 420 – 500 650-710
Cy2/Cy5 2 470, 561, 633, 640 LP 620 485 – 550 650-710
CFP 440/YFP 2 405, 440, 514 LP 505 465-500 535-585
CFP 440/HcRed 2 405, 440, 514, 543,
561
LP 560 465-500 581-654
empty 1 - - -
VIS 1 405 - - LP 430
DAPI 1 405 - - 420 - 500
FITC 1 405, 470, 488 - - 500 - 550
TRITC 1 514, 543 - - 565 - 605
Cy5 1 562, 594, 633 - - 650 - 710