Leica TCS SP8 SMD, User Manual

Page: 143 / 270

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FLIM Data Acquisition
Recommended settings of the fluorifier disc for SP FLIM detection:
Table 19: Recommended settings for Fluorifier disc for SP FLIM
As long as the Fluorifier Disc Auto Select is active in the dialog window, the position of the
Fluorifier disc is automatically set. It can be changed by the user if Auto Select is disabled.
To do so, proceed as follows:
1. In Beam Path Settings , click on the Fluorifier Disc button ( Figure 125 ).
Figure 125: Fluorifier Disc button in Beam Path Settings
The following dialog box opens:
Figure 126: Fluorifier Disc Settings
Excitation laser line Recommended setting of the fluorifier disc
1 405 Barrier filter 405/640
2 405+470 Barrier filter 405/470
3 405+ 640 Barrier filter 405/640
4 470 Barrier filter 405/470
5 470+640 Barrier filter 470/640
6 640 Barrier filter 405/640 or 470/640
7 MP SP680 or SP700
8 WLL Depending on the selected white light laser wavelength,
there are barrier filters for:
488, 514, 561, 594, or 633