Dionex AS-HV, Operator'S Manual

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AS-HV Operator’s Manual
82
Doc. 065125-01 10/06
B.3.17 Removing Bubbles from the Syringe
Disregard this section if a syringe pump is not installed.
1. Fill a container with isopropyl alcohol.
2. Unscrew and remove the
plunger lock screw (see
Figure B-15 ).
3. Hold the syringe at the top and
bottom and unscrew it from
the valve.
4. Pull the syringe plunger up and
push down rapidly with short
movements to dislodge the
bubbles. Repeat until all
bubbles are dislodged.
5. Place the threaded end of the
syringe into the isopropyl
alcohol and slowly pull out the
plunger to draw a full syringe
volume into the syringe.
Figure B-15. Removing the Syringe
6. Keeping the syringe in the liquid, slowly push the syringe plunger
until the bubble(s) exits the syringe. Then, fill the syringe.
7. Alternatively, fill the syringe with isopropyl alcohol and then remove
the syringe from the liquid. Hold the syringe with the threaded end up
under a stream of deionized water. Push the syringe plunger up and
pull down until the bubble is removed.
8. Remove the syringe from the liquid. While holding it vertically, push
a small amount of liquid out the top to make sure there is no air
present.
9. Position the syringe under the valve.
Remove the plunger lock
screw
Valve
Syringe