Biotek Synergy H1, Operator'S Manual

Page: 71 / 190

Figure 4-2: Proper orientation of the EX (left) and EM (right)
filters in the filter cube
The filter cube can hold up to two half-size, or one full-size, dichroic or 50% mirror. The
mirror positions are labeled “1”and “2” to coordinate with EX1/EM1 and EX2/EM2.
Mirror direction is also important. The mirror label should be in the lower-right corner of
the mirror and readable (see Figure 4-3 on the next page). If the mirror is positioned
incorrectly, your measurement data may be inaccurate.
For Synergy H1 models with FP capability, the cube is equipped with up to four polarizers
of the following types:
l
Excitation polarizer (visible-range or UV-range)
l
Emission polarizer, parallel to the excitation polarizer
l
Emission polarizer, perpendicular to the excitation polarizer
Two types of excitation (EX) polarizers are available: visible-range (400 nm and above, the
default) or UV-range (300 nm and above, available from BioTek). The polarizers, if used,
are placed below the excitation filters and above the emission filters. The polarizer filters
are keyed to fit in the correct alignment in the filter cube.
Synergy H1 Operator's Manual
Filter Cube Overview | 45