Fluorescence Liquid Tests
starting on page
describes the test methods, lists the Gen5
protocol parameters, explains how to analyze the test results, and provides
troubleshooting tips in the event of test failure.
The tests in this section require specific microplates, solutions, and
filters or wavelengths. Your laboratory may require a deviation from
some of these tests. For example, you may wish to use a different plate
or test solution. If deviation from the tests as presented in this section is
required, perform the following steps the first time each test is run:
Perform the tests exactly as described here.
Rerun the tests using your particular plates, solutions, and so on.
If results are comparable, then the results from these tests will be
your baseline for future tests. Document your new test procedure
and save all test results.
Materials
Kits containing the microplates and solutions required by the Liquid Tests
are available for purchase; see
Materials for Conducting Liquid Tests
on
page
.
i
Microplates should be clean and free from dust and bottom scratches. Use new
microplates from sealed packages.
Manufacturer part numbers are subject to change.
All tests:
l
Deionized or distilled water
l
Various beakers, graduated cylinders, and pipettes
l
95% ethanol (for cleaning clear-bottom plates)
l
Aluminum foil
l
(Optional, but recommended) 0.45-micron filter
l
(Optional) Black polyethylene bag(s) to temporarily store plate(s)
Synergy H1 Operator's Manual
Fluorescence Liquid Tests | 121
Summary of Contents for Synergy H1
Page 1: ...Hybrid Multi Mode Microplate Reader Synergy H1 TM Operator s Manual ...
Page 2: ......
Page 26: ...BioTek Instruments Inc xxiv Preface ...
Page 54: ...BioTek Instruments Inc 28 Chapter 2 Installation ...
Page 68: ...BioTek Instruments Inc 42 Chapter 3 Getting Started ...
Page 132: ...BioTek Instruments Inc 106 Chapter 7 Instrument Qualification Process ...