Biotek Synergy H1, Operator'S Manual

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Absorbance Testing
For models with absorbance capability, BioTek developed a series of tests for the
absorbance system using a combination of solid state Absorbance Test Plates and liquid
plates. The test plates and the materials used for creating the liquid plates are available for
purchase from BioTek.
To qualify the absorbance system for the Synergy H1, you should perform:
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Absorbance Liquid Test 1 and Absorbance Plate Test (using BTI #7260522) or
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Absorbance Liquid Test 2
Optionally, to qualify operation in the UV range, you should also perform:
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Absorbance Liquid Test 3 or Absorbance Plate Test at 340 nm (using BTI #7260551)
BioTek Absorbance Test Plates
Absorbance Test Plate PN 7260522 uses NIST-traceable neutral density filters to confirm
absorbance specifications in the visible range (400–800 nm). This test plate also contains
precision-machined holes to verify mechanical alignment, and a glass filter in position C6
to test the wavelength accuracy of the monochromator-based absorbance system.
Absorbance Test Plate PN 7260551 uses NIST-traceable neutral density filters to confirm
absorbance specifications in the UV range (340 nm).
Every test plate comes with a Test Plate Calibration Certificate, containing a table with
Absorbance OD Standards for each filter at each wavelength supported by the plate. The
certificate for test plate PN 7260522 also contains Wavelength Accuracy Standards tables
with Expected Peak (nm) values with Test Ranges for the C6 glass filter.
Before the Absorbance Plate Test can be performed, the OD Standard values and Expected
Peak/Test Range combinations must be entered into Gen5. Enter and save these values
once initially, and then update them annually when the test plate is recertified by BioTek.
Test Methods
The Absorbance Plate Test is conducted using Gen5 software (System > Diagnostics
> Test Plates) to confirm wavelength accuracy ("Peak Absorbance"); mechanical
alignment; and optical density accuracy, linearity, and repeatability. When complete,
Gen5 generates a results report displaying Pass or Fail for each individual test.
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Peak Absorbance: The BTI #7260522 test plate contains a glass filter in position C6
that is used to check the wavelength accuracy of the absorbance monochromator.
The filter is scanned across a specified wavelength range in 1-nm increments. The
wavelength(s) of maximum absorbance are compared to the expected peak
wavelength(s) supplied on the test plate’s data sheet. The accuracy of the
wavelength should be ± 3 nm (±2 nm instrument, ±1 nm filter allowance).
Synergy H1 Operator's Manual
Absorbance Testing | 75