Biotek Synergy H1, Operator'S Manual

Page: 60 / 190

4 Outlet tubes transport fluid from the syringes into the instrument, through the
tubing ports on the reader's top cover. The outlet tubes are opaque PTFE tubes
with threaded fittings on each end.
Avoid continuous contact with harsh chemicals. Rinse the fluid path with
deionized water after contact with any strong acid, base, or solvent.
For information on the materials used in the injection system, refer to
Injection System - Chemical Compatibility Technical Note on the
USB flash drive supplied with the Synergy H1.
Priming the Injection System
Before running a Dispense assay, use Gen5 to prime the system with the reagent or
dispensing fluid. An additional tip prime can be performed at the start of the assay and,
sometimes, just before each dispense to a well. The tip prime compensates for any fluid
loss at the injector tip due to evaporation since the last dispense. All priming activities
are controlled using Gen5 (see
Dispense Module Control on page 37 ).
If the injection system is not adequately primed, air bubbles can get
trapped in the system and affect injection volumes. Air bubbles in the
system can also result in fluid spraying or scattering inside the reader.
Both types of primes require a fluid reservoir to be present on the microplate carrier.
See the photo in
Test the Injection System on page 20 .
l
The priming plate is placed on the microplate carrier for a Prime operation (to
prime the dispense system with fluid).
l
The tip priming trough is placed in the rear pocket of the carrier, and is used for
performing the Tip Prime before dispensing. The trough holds up to 1.5 mL of
liquid and must be periodically emptied and cleaned by the user.
Do not perform tip priming when using tall plates. Generally, plates with
fewer than 96 wells are too tall for error-free tip priming; and, tip
priming is rarely required for these larger-volume plates.
The priming plate should be empty before priming, and it should contain
fluid after priming.
34 | Chapter 3: Getting Started
BioTek Instruments, Inc.