Biotek Synergy H1, Operator'S Manual

Page: 131 / 190

Parameter Setting
Read Step Detection Method: Absorbance
Read Type: Endpoint
Optics Type: <as appropriate for the reader type>
Step label: 20 and 5 ul Read
Wells: A5..H12
Wavelengths, 2: 630 nm, 750 nm
Speed: Normal
Data Reduction Define two Delta OD transformations:
405-750 nm for the 80 ul Read step, columns 1-4
630-750 nm for the 20 and 5 ul Read step, columns 5-12
Results Analysis
The Injection System Test procedures begin on page 134 .
When the experiment for one injector is complete, 32 delta OD values are reported for
each of the three dispense volumes. The pass/fail criteria for each set of 32 wells with the
same dispense volume is based on the calculated coefficient of variation (% CV) and
Accuracy % Error.
For each volume dispensed (80 μL, 20 μL, 5 μL), for each injector (1, 2):
1. Calculate the Standard Deviation of the 32 wells
2. Calculate the Mean of the 32 wells
3. Calculate the %CV: (Standard Deviation / Mean) x 100
4. Calculate the Accuracy % Error:
((ActualWeight–ExpectedWeight)/ExpectedWeight)* 100
Expected Weights for 32 wells: 80 μL (2.560 g), 20 μL (0.640 g), 5 μL (0.160 g).
It is assumed that one gram is equal to one milliliter.
Dispense Volume To pass, %CV
must be:
To pass, Accuracy % Error
must be:
80 µL ≤ 2.0% ≤ 2.0%
20 µL ≤ 7.0% ≤ 5.0%
5 µL ≤ 10.0% ≤ 20.0%
If any tests fail, prime the fluid lines and rerun the tests. If the tests fail again, the
injectors may require cleaning; see
Clean the Dispense Tubes and Injectors on page 63 .
If tests continue to fail, contact BioTek TAC.
Synergy H1 Operator's Manual
Injection System Testing | 105