Biotek Synergy H1, Operator'S Manual

Page: 62 / 190

5. Select the mirror type and enter the excitation and emission ranges. (Note that the
"M" value on the filter cube label is the cut-off (nm). Refer to the mirror information
table on page
48 .)
i If 'Fluorescence Polarization Cube' is checked, only Filter Set 1 is required
for definition. The filters and mirrors of Filter Set 2 must be identical to those of
Filter Set 1 for FP.
6. Define Filter Set 2, if necessary.
7. Click Send Values to transfer the information to the reader.
8. When finished, click Close .
Protocols and Experiments
In Gen5, a protocol contains instructions for controlling the reader and (optionally) for
analyzing data retrieved from the reader. At a minimum, a protocol must specify the
procedure for the assay you wish to run. After creating a protocol, create an experiment
that references the protocol. You’ll run the experiment to read plates and analyze the data.
These instructions briefly describe how to create a protocol in Gen5. See the Gen5 Help
system for complete instructions.
1. In the Gen5 Task Manager, select the Protocols icon and click Create New .
2. Open the Procedure dialog (double-click Procedure in the menu tree).
3. Select an appropriate Plate Type.
Gen5 stores measurements and other characteristics for individual plate
types in a database. It is essential that you select (or define) the plate
type to match the assay plate. Otherwise,
results may be invalid .
See the "Plate Type Database" topic in the Gen5 Help for instructions.
4. Add steps to the procedure to shake or heat the plate, dispense fluid, read the plate,
and more.
5. Click Validate to verify that the attached reader supports the defined steps, and
then close the Procedure dialog.
6. Optionally, perform any of these steps to analyze and report the results:
l
Open the Plate Layout dialog and assign blanks, samples, controls, and/or
standards to the plate.
l
Open the Data Reduction dialog to add data reduction steps. Categories include
Transformations, Well Analysis, Curve Analysis, and Qualitative Analysis.
l
Create a report or export template via the Report/Export Builders.
7. Select File > Save and give the protocol an identifying name.
36 | Chapter 3: Getting Started
BioTek Instruments, Inc.