Biotek Synergy H1, Operator'S Manual

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Code Description and possible remedy
4Fxy Fluorescence signal out of range
x=0, 1; y=0–6
Verify that the Gen5 Fluor/Lum wavelengths table matches the
actual filter installed in the filter cube. Verify that there is no filter
wavelength overlap between the emission/excitation positions.
Verify that the microplate door is fully closing, and the instrument
cover is properly installed and sealed.
Try lowering the Gain in your Read step(s).
The reading chamber may be contaminated by a spill that is
fluorescing; see the
Preventive Maintenance chapter.
5003/5103 Filter cube did not home
Generally, this error indicates the filter cube is not seated properly
in the reader. Remove it, ensure each filter or plug is properly
positioned and reinstall it securely. Restart the reader.
5403 Filter cube failed positional verify
Generally, this error indicates the filter cube is not seated properly
in the reader. Remove it, ensure each filter or plug is properly
positioned and reinstall it securely. Restart the reader.
55xy <Motor> not homed successfully
xy=axis
This error indicates that an axis failed a previous verify function
and now needs to be homed. Verify that the shipping brackets have
been removed. Check for any obstructions that may prevent the
carrier, syringes, or filter cube from moving normally. Restart the
reader.
Synergy H1 Operator's Manual
Error Codes | 149