Biotek Synergy H1, Operator'S Manual

Page: 138 / 190

Absorbance Plate Tests
BioTek Absorbance Test Plates starting on page 75 describes the test methods and
provides troubleshooting tips in the event of test failure.
i
The diagnostics feature in Gen5 versions 2.08 and higher is compatible with the 340
nm Absorbance Test Plate BTI #7260551. If you are using an earlier Gen5 version, refer
to the test plate's instruction sheet to manually conduct the tests and analyze results.
Requirements
To perform this test, you will need:
l
Absorbance Test Plate, BTI #7260522
l
(Optional) 340 nm Absorbance Test Plate, BTI #7260551
l
Current Absorbance Test Plate Calibration Certificate(s)
Setup
Before an Absorbance Test Plate can be used for qualification, you must enter information
from its Calibration Certificate into Gen5. Perform these steps initially, and then repeat
them annually after the test plate is recertified by BioTek:
1. Obtain the current Test Plate Calibration Certificate.
2. Start Gen5 and select System > Diagnostics > Test Plates > Add/Modify
Plates .
3. Click Add . The Absorbance Test Plate dialog appears.
4. Select the appropriate Plate Type and then enter the plate’s serial number.
5. Enter the Last Certification and Next Certification dates from the calibration label on
the Test Plate.
6. If the wavelength values in the top row of the grid in Gen5 are appropriate for your
tests, enter the OD Standard values from the Calibration Certificate into the grid.
Make sure you enter the correct value for each well/wavelength combination.
If you need to change the wavelength values, click Wavelength List . Add,
change, or delete the values as needed and click OK .
7. If applicable: Select the number of Peak Wavelength tests to run (up to 4), based on
the desired Expected Peak wavelengths provided on the Calibration Certificate. Enter
the Expected Peak value(s) from the Certificate and set the Test Range – and +
values.
112 | Chapter 8: Instrument Qualification Procedures
BioTek Instruments, Inc.