Biotek Synergy H1, Operator'S Manual

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Pipette Map
Using a single-channel pipette:
l
Pipette 200 µL of the 17.6 ng/mL (100 nM) MUB solution into well D7.
l
Pipette 200 µL of the buffer solution into wells C9, D9, and E9.
Using a multi-channel pipette with just four tips installed:
l
Pipette 150 μL of the buffer into wells C2-F5. Discard the tips.
l
Pipette 150 μL of the 17.6 ng/mL (100 nM) MUB solution into column 1.
l
Pipette 150 μL of the 17.6 ng/mL (100 nM) MUB solution into column 2. Mix the wells
using the pipette. Do not discard the tips.
l
Aspirate 150 μL from column 2 and dispense into column 3. Mix the wells using the
pipette. Do not discard the tips.
l
Aspirate 150 μL from column 3 and dispense into column 4. Mix the wells using the
pipette. Do not discard the tips.
l
Aspirate 150 μL from column 4 and dispense into column 5. Mix the wells using the
pipette. Do not discard the tips.
l
Aspirate 150 μL from column 5. Discard the solution and the tips.
1 2 3 4 5 6 7 8 9 10 11 12
A
B
C 100 nM 50 nM 25 nM 12.5 nM 6.25 nM BUF
D 100 nM 50 nM 25 nM 12.5 nM 6.25 nM MUB
100 nM
BUF
E 100 nM 50 nM 25 nM 12.5 nM 6.25 nM BUF
F 100 nM 50 nM 25 nM 12.5 nM 6.25 nM
G
H
Synergy H1 Operator's Manual
Fluorescence Liquid Tests | 133