Biotek Synergy H1, Operator'S Manual

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Pipette Maps
Seal the plates with foil or store them in black polyethylene bags until use. When using a
clear-bottom plate, if the base of the plate is touched, clean the entire base with alcohol
(95% ethanol) and then wipe with a lint-free cloth. Before placing the plate in the
instrument, blow the bottom of the plate with an aerosol duster.
Corners, Sensitivity, and Linearity (FI) Tests:
Refer to the illustration on the next page.
Using a single-channel pipette:
l
Pipette 200 μL of the 3.3 nM SF solution into the “corner” wells.
l
Pipette 200 μL of the buffer in the wells surrounding the SF. (Omit if using a solid
black plate or Greiner SensoPlate.)
l
Pipette 200 µL of the 1 nM SF solution into well D7.
l
Pipette 200 µL of the buffer solution into wells C9, D9, and E9.
Using a multi-channel pipette with just four tips installed:
l
Pipette 150 μL of the buffer into wells C2-F5. Discard the tips.
l
Pipette 150 μL of the 1 nM SF solution into column 1.
l
Pipette 150 μL of the 1 nm SF solution into column 2. Mix the wells using the pipette.
Do not discard the tips.
l
Aspirate 150 μL from column 2 and dispense into column 3. Mix the wells using the
pipette. Do not discard the tips.
l
Aspirate 150 μL from column 3 and dispense into column 4. Mix the wells using the
pipette. Do not discard the tips.
l
Aspirate 150 μL from column 4 and dispense into column 5. Mix the wells using the
pipette. Do not discard the tips.
l
Aspirate 150 μL from column 5. Discard the solution and the tips.
Synergy H1 Operator's Manual
Fluorescence Liquid Tests | 127