Biotek Synergy H1, Operator'S Manual

Page: 164 / 190

of the green test dye solution.
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Carefully set the plate aside.
9. Close the experiment without saving it.
If you are not using a BioTek absorbance reader, read the plate using the
parameters described in the table above. Perform the calculations and
dettermine pass/fail according to the instructions on page
105 .
10. If you are using a BioTek absorbance reader, configure Gen5 to communicate with
the reader.
11. Create an experiment based on the Other Reader protocol and read the plate.
12. When the experiment is complete, save the file with an identifying name.
13. Remove the plate from the carrier and set it aside.
14. Repeat the procedure using the Synergy H1 Disp 2 Test No Read protocol and a
new microplate.
15. When the tests are complete:
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Prime both dispensers with at least 5000 µL of deionized water to flush out the dye
solution.
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Refer to the instructions on page 105 to perform calculations and determine
pass/fail.
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Test descriptions are provided on page 102 .
138 | Chapter 8: Instrument Qualification Procedures
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