Biotek Synergy H1, Operator'S Manual

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Injection System Testing
For models equipped with injectors and an external dispense module, BioTek has
developed a set of tests to ensure that the injection system performs to specification.
Test Method
The Accuracy Test is a measure of the mean volume per well for multiple dispenses. The
actual weight of the dispensed fluid is compared to the expected weight and must be within
a certain percentage to pass. Pass/Fail criteria depends on the per-well volume dispensed:
2.0% for 80 μL, 5.0% for 20 μL, and 20.0% for 5 μL.
The test uses a green dye test solution (available for purchase from BioTek, see page 5 )
and one 96-well microplate per injector to test the three different volumes. The balance is
tared with the empty plate and the 80 μL dispense is performed for columns 1–4. The fluid
is weighed and the balance is tared again with the plate. This process is repeated for the 20
μL and 5 μL dispenses.
It is assumed that one gram is equal to one milliliter and the solutions used are at room
temperature. A three-place precision balance is used to weigh the plate.
The Precision Test is a measure of the variation among volumes dispensed to multiple
wells, and uses the green test dye solution. For each volume dispensed (80 μL, 20 μL, and
5 μL) to four columns, the %CV of 32 absorbance readings is calculated. Pass/Fail criteria
depends on the per-well volume dispensed: 2.0% for 80 μL, 7.0% for 20 μL, and 10.0% for
5 μL. Columns 1–4 are read at 405/750 nm and columns 5–12 at 630/750 nm.
The Accuracy and Precision tests are performed simultaneously and use the same plate.
Gen5 Parameters
The information in this section represents the recommended reading parameters for the
referenced Gen5 protocol(s). It is possible that your tests will require modifications to
some of these parameters, such as the Plate Type.
i The Plate Type setting in each Gen5 protocol should match the actual plate in use.
Synergy H1 Disp 1 Test.prt and Synergy H1 Disp 2 Test.prt (for use with models
with Absorbance capability)
Parameter Setting
Plate Type 96 WELL PLATE
Dispense Step Dispenser <1 or 2>
Wells A1–H4
Tip prime before this dispense step, 20 µL
Dispense 80 µL at 275 µL/sec
102 | Chapter 7: Instrument Qualification Process
BioTek Instruments, Inc.