Biotek Synergy H1, Operator'S Manual

Page: 130 / 190

Synergy H1 Disp 1 Test No Read.prt and Synergy H1 Disp 2 Test No Read.prt (for
use with models without Absorbance capability)
Parameter Setting
Plate Type 96 WELL PLATE
Dispense Step Dispenser <1 or 2>
Wells A1..H4
Tip prime before this dispense step, 20 µL
Dispense 80 µL at 275 µL/sec
Plate Out,In Comment: Weigh the plate (80 uL test). RECORD the weight, TARE
the balance. Place the plate back on the carrier. Click OK to
continue.
Dispense Step Dispenser <1 or 2>
Wells A5..H8
Tip prime before this dispense step, 20 µL
Dispense 20 µL at 250 µL/sec
Plate Out,In Comment: Weigh the plate (20 uL test). RECORD the weight, TARE
the balance. Place the plate back on the carrier. Click OK to
continue.
Dispense Step Dispenser <1 or 2>
Wells A9..H12
Tip prime before this dispense step, 5 µL
Dispense 5 µL at 225 µL/sec
Plate Out,In Comment: Weigh the plate (5 uL test). RECORD the weight, TARE
the balance. PIPETTE 150 µL/well of DI water into all 12 columns.
Set the plate aside and click OK.
Read Step Define a brief Read step for a single well. The measurement value
will not be used. The step is only necessary because Gen5 requires
a Read step with dispense protocols.
Synergy H1 Disp Test Other Reader.prt (for use with a BioTek absorbance-capable
reader other than Synergy H1)
Parameter Setting
Shake Step <medium intensity> for 15 seconds
Read Step Detection Method: Absorbance
Read Type: Endpoint
Optics Type: <as appropriate for the reader type>
Step label: 80 ul Read
Wells: A1..H4
Wavelengths, 2: 405 nm, 750 nm
Speed: Normal
104 | Chapter 7: Instrument Qualification Process
BioTek Instruments, Inc.