Biotek Synergy H1, Operator'S Manual

Page: 46 / 190

14: Test the Injection System
Applies only to models equipped with injectors
1. If necessary, press the carrier eject button to eject the microplate carrier.
2. Place the tip priming trough in its pocket in the carrier.
3. Place the priming plate on the carrier.
Figure 2-6: Priming trough and plate installed on the carrier
4. Fill the two reagent bottles with distilled or deionized water. Place the bottles in their
holders, and place the holders directly in front of the syringes. Insert the inlet tubes
into the bottles.
5. In Gen5, select System > Instrument Control > Synergy H1 and click the
Prime tab.
6. With Dispenser set to 1, set the Volume to 5000 µL and click Prime . The syringe
should move down and up repeatedly, drawing fluid from the bottle and pumping it
through the tubing and into the priming plate. Examine the fittings; no leaks should
be detected. If leaks are detected, tighten all fittings and repeat the prime. If leaks
are still detected, contact BioTek’s Technical Assistance Center.
7. When finished, set the Volume to 2000 µL and click Purge to clear the fluid lines.
8. Set the Dispenser to 2 and repeat steps 6 and 7.
9. Remove and empty the priming plate.
20 | Chapter 2: Installation
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