Biotek Synergy H1, Operator'S Manual

Page: 73 / 190

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Figure 4-4: Filter cube sliding into the chamber (left) and
properly seated (right)
Configuring the System for Luminescence Measurements
If your tests require that the light emitted from the samples remain
unfiltered, the Emission filter position in the filter cube should be empty.
As discussed on page
35 , if you make any changes to the filter cube, you
must update the Gen5 Filter Cube table; select HOLE next to Emission to
indicate the empty location.
About the Gen5 Optics Library
Gen5 provides an option to create a "library" of the filter cubes used with your assays.
You'll define a name and characteristics for each cube, reference the desired cube by name
in a protocol, and then update the reader's internal software to match the currently-
installed filter cube by selecting
System > Optics Library > Set Reader . You can also
enable a Read Plate Prompt option to alert users at run-time if they attempt to run an
experiment that calls for filters/mirrors not currently installed. Brief instructions for adding
a filter cube to the library are provided below; refer to the Gen5 help system to learn
more.
Adding a Filter Cube to the Gen5 Optics Library
1. From the Gen5 main view, select System > Optics Library > Filter Cubes .
2. Click Add and enter a name for the filter cube. This name will become available for
selection in the protocol/experiment procedure.
3. If applicable , check the Fluorescence Polarization Cube box.
4. Enter a name for Filter Set 1.
5. Define the excitation and emission filters:
Synergy H1 Operator's Manual
Filter Cube Overview | 47