Biotek Synergy H1, Operator'S Manual

Page: 61 / 190

Gen5 Software
Gen5 supports all Synergy H1 models. Use Gen5 to control the reader, the dispense module
(if equipped), and the BioStack (if equipped); perform data reduction and analysis on the
measurement values; print or export results; and more. This section provides brief
instructions for working with Gen5 to create protocols and experiments and read plates.
Refer to the
Gen5 Getting Started Guide and the Help system for more information.
Define the Filter Cube in Gen5 and on the Reader
As described on page 32 , most Synergy H1 models are delivered with a filter cube
installed, and the reader’s onboard software is configured with the filter and mirror values
and their locations in that filter cube. When Gen5 communicates with the reader, it “asks”
for this information and then stores the values in a Filter Cube table.
Important! It is critical that the values in Gen5 and the reader's
software exactly match the contents of the installed filter cube.
If you exchange or modify the filter cube, you must update the Gen5 Filter Cube table and
send the information to the reader:
1. From the Gen5 main view, select System > Instrument Configuration . Highlight
the Synergy H1 , click View/Modify and then click Setup .
2. If this is a new filter cube, enter a unique name to identify the cube and then enter a
name for Filter Set 1.
3. If applicable , check the Fluorescence Polarization Cube box.
4. Define/modify settings for the excitation and emission filters:
l
Select Band Pass , Long Pass , or Short Pass , as appropriate for each filter
type.
Band Pass a standard interference filter with a defined central
wavelength and bandwidth
Long Pass cutoff filter that transmits longer wavelengths and block
shorter wavelengths
Short Pass cutoff filter that transmits shorter wavelengths and
block longer wavelengths
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Select PLUG to indicate the presence of a plug.
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Select HOLE to indicate an empty location.
Synergy H1 Operator's Manual
Gen5 Software | 35