Biotek Synergy H1, Operator'S Manual

Page: 161 / 190

Alternate Test Solutions
If you do not have BioTek’s Green Test Dye Solution (PN 7773003), prepare a dye
solution using one of the following methods:
80 μL of test solution with 150 μL of deionized water should read between 1.300
and 1.700 OD at 405/750 nm. The solutions should be at room temperature.
Using BioTek's Blue and Yellow Concentrate Dye Solutions:
Item Quantity
Concentrate Blue Dye Solution
(BTI #7773001, 125 mL) 4.0 mL
QC (Yellow) Solution
(BTI #7120782, 125 mL) 5.0 mL
Deionized water 90.0 mL
Using FD&C Blue and Yellow Dye Powder:
Item Quantity per Liter
FD&C Blue No. 1 0.200 grams
FD&C Yellow No. 5 0.092 grams
Tween 20 1.0 mL
Sodium Azide N
3
Na 0.100 gram
Deionized water make to 1 liter
Test Procedure for Models with Absorbance Capability
1. Prime both dispensers with 4000 μL of deionized or distilled water.
2. Remove the inlet tubes from the supply bottles. Prime both dispensers with the
Volume set to 2000 μL. This prevents the water from diluting the dye.
3. Fill a beaker with at least 20 mL of the green dye solution. Prime both dispensers
with 2000 μL of the solution. When finished, remove the priming plate from the
carrier.
4. Create an experiment based on the Synergy H1 Disp 1 Test protocol.
5. Place a new 96-well microplate on the balance and tare the balance.
6. Place the plate on the microplate carrier.
Running a dispense protocol with no plate on the carrier will
contaminate the reading chamber with spilled fluid.
Synergy H1 Operator's Manual
Injection System Tests | 135