Biotek Synergy H1, Operator'S Manual

Page: 88 / 190

Run a Dispense Protocol (Optional)
Applies only to models equipped with injectors
After flushing/purging the system (described on page 61 ) and before running an assay that
requires dispense, take a moment to visually inspect the dispense accuracy.
Use a DI H
2
O–Tween solution to visually inspect the dispense accuracy
following maintenance: e.g., add 1 mL Tween 20 to 1000 mL of deionized
water.
1. Create a new protocol in Gen5. Select a Plate Type that matches the plate you are
using.
2. Add a Dispense step with the following parameters:
l
Select Dispenser 1
l
Set Tip Priming to "Before this dispense step" and Volume to 10 µL
l
Set the Dispense Volume to 100 µL (or an amount to match your assay protocol)
l
Adjust the Rate to support the dispensing volume
3. Add another Dispense step with the same parameters, selecting Dispenser 2.
4. Add a quick Read step with parameters relevant to your reader model (this is
necessary because Gen5 requires that a Read step follow the Dispense step).
5. Save the protocol with an identifying name, such as “Dispense Observation.”
6. Fill the supply bottles with the DI H
2
O–Tween solution mentioned above.
7. Create and run an experiment based on the Dispense Observation protocol.
8. When the experiment is complete, visually assess the fluid level in the wells. Well
volumes should appear evenly distributed across the plate.
If the well volume appears to be unevenly distributed, clean the internal dispense
tubes and injectors as described in
Clean the Dispense Tubes and Injectors starting
on page 63 and run the protocol again.
62 | Chapter 5: Preventive Maintenance
BioTek Instruments, Inc.