Biotek Synergy H1, Operator'S Manual

Page: 154 / 190

1 2 3 4 5 6 7 8 9 10 11 12
Corners
A 3.3 nM 3.3
nM
3.3 nM BUF BUF 3.3
nM
3.3
nM
3.3
nM
B BUF BUF BUF BUF BUF BUF BUF BUF
C 150
µL:
1 nM
0.5
nM
0.25
nM
0.125
nM
0.0625
nM
BUF
Sensitivity
/Linearity
D
1 nM
0.5
nM
0.25
nM
0.125
nM
0.0625
nM
200
µL:
1 nM
BUF
E 1 nM 0.5
nM
0.25
nM
0.125
nM
0.0625
nM
BUF
F 1 nM 0.5
nM
0.25
nM
0.125
nM
0.0625
nM
G BUF BUF BUF BUF BUF BUF BUF BUF
Corners H 3.3 nM 3.3
nM
3.3 nM BUF BUF 3.3
nM
3.3
nM
3.3
nM
Fluorescence Polarization (FP) Test:
The plate used for testing Corners/Sensitivity/Linearity of the Top optics can
also be used for this test.
l
Pipette 200 μL of the (green) polarization buffer (BUF) into wells A6–H6.
l
Pipette 200 μL of the green high polarization reference (HPR) into wells A7–B7.
l
Pipette 200 μL of the green low polarization reference (LPR) into wells A8–H8.
1 2 3 4 5 6 7 8 9 10 11 12
A BUF HPR LPR
B BUF HPR LPR
C BUF LPR
D BUF LPR
E BUF LPR
F BUF LPR
G BUF LPR
H BUF LPR
128 | Chapter 8: Instrument Qualification Procedures
BioTek Instruments, Inc.