Biotek Synergy H1, Operator'S Manual

Page: 127 / 190

Troubleshooting
If a fluorescence liquid test fails, try the relevant suggestions below. If a test continues
to fail, print the results and contact BioTek TAC.
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Are the solutions fresh? Discard open/unused buffer and stock solutions after seven
days.
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Are the Excitation/Emission filters clean? Are they in the proper locations and in the
proper orientation in the filter cube or wheel?
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Are you using new/clean plates? If the base of a clear-bottom plate is touched, clean
the entire base with alcohol (95% ethanol) and then wipe with a lint-free cloth.
Before placing the plate in the instrument, blow the bottom of the plate with an
aerosol duster. If the test fails again, the optical probe(s) may need to be cleaned.
Contact BioTek TAC for instructions.
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Review the pipetting instructions to verify the plate was correctly prepared.
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Does the Plate Type setting in the Gen5 protocol match the plate you used?
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For injector models, spilled fluid inside the reader may be fluorescing, which can
corrupt your test results. If you suspect this is a problem, contact BioTek TAC for
assistance.
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When testing Fluorescence Polarization capability using a solid black plastic
microplate, if the standard deviation for the buffer wells is too high, try either
moving the buffer wells to another column, or using the Greiner SensoPlate. With
some black plastic plates, the wells in the center of the plate may be slightly
distorted due to the plate molding process, and this can affect the standard deviation.
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If the Corners Test continues to fail, the hardware may be misaligned. Contact
BioTek TAC.
Synergy H1 Operator's Manual
Fluorescence Testing | 101