Biotek Synergy H1, Operator'S Manual

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Filter Cube Overview
Most Synergy H1 models are equipped with a filter cube that contains excitation filters,
emission filters, and mirrors for use when taking fluorescence and luminescence
measurements. Each filter cube contains two filter sets, each of which contains one
excitation filter, one emission filter, and one mirror. The filter cube is accessed through a
hinged door in the front of the instrument. Each filter has an ID label for recording the
cube's contents.
Figure 4-1: Synergy H1 filter cube
You can easily exchange one filter cube with another to meet varying assay requirements.
If you regularly need to use different filters or mirrors, consider purchasing additional filter
cubes from BioTek.
Use the Gen5 Optics Library to identify and manage the contents of multiple
filter cubes; see
About the Gen5 Optics Library on page 47 .
The default filter cube configuration is shown below; any changes are reflected in the sales
order. Verify that the filter cube contains the filters and mirrors that you ordered. Contact
BioTek or your supplier if you did not receive the expected components.
Position 1 Position 2
Excitation 360/40 nm 485/20 nm
Emission 460/40 nm 528/20 nm
Mirror 400 nm 510 nm
Filters are not specific to either excitation or emission. Filter direction within the filter cube
is important, and as illustrated in Figure 4-2 , the direction differs depending on the filter's
placement in the cube (EX or EM). Each filter has its central wavelength and bandpass
values printed on its side, with an arrow to indicate the proper direction of light through the
filter.
44 | Chapter 4: Filters and Mirrors
BioTek Instruments, Inc.