Biotek Synergy H1, Operator'S Manual

Page: 169 / 190

Absorbance Specifications
Optics
Wavelength Range 230 to 999 nm
Wavelength Bandpass <4 nm (230–285 nm), <8 nm (>285 nm)
Measurement Range 0.000 to 4.000 OD
Resolution 0.001 OD
Increment 1 nm
Wavelength Accuracy ±2 nm
Wavelength Precision 0.2 nm (standard deviation)
Wavelength Repeatability ± 0.2 nm
Performance
Specifications apply from 250–999 nm
Accuracy
Using certified neutral density glass
96-well plate, normal read speed
0–2 OD: ±1% ±0.010 OD, delay after plate movement=100 ms
2–2.5 OD: ±3% ±0.010 OD, delay after plate movement=100 ms
384-well plate, normal read speed
0–2 OD: ±2% ±0.010 OD, delay after plate movement=100 ms
2–2.5 OD: ±5% ±0.010 OD, delay after plate movement=100 ms
96-well and 384-well plate, sweep read speed
0–1 OD: ±1% ±0.010 OD
Linearity
By liquid dilution
96-well plate, normal read speed
0–2 OD: ±1% ±0.010 OD, delay after plate movement=100 ms
2–2.5 OD: ±3% ±0.010 OD, delay after plate movement=100 ms
384-well plate, normal read speed
0–2 OD: ±2% ±0.010 OD, delay after plate movement=100 ms
2–2.5 OD: ±5% ±0.010 OD, delay after plate movement=100 ms
Synergy H1 Operator's Manual
Absorbance Specifications | 141