Biotek Synergy H1, Operator'S Manual

Page: 87 / 190

Flush/Purge Fluid Path
Applies only to models equipped with injectors
At the end of each day that the dispense module is in use, flush the fluid path using the
Gen5 priming utility. Leave the fluid to soak overnight or over a weekend, and then purge
the fluid before using the instrument again.
This flushing and purging routine is also recommended before disconnecting the outlet
tubes from the reader, and before decontamination to remove any assay residue prior
to applying isopropyl alcohol or sodium hypochlorite.
i If using Gen5 version 2.05 or earlier, the Prime and Purge options are found under
the Dispenser tab in the Reader Control dialog.
To flush the fluid path:
1. Fill two supply bottles with deionized or distilled water. Insert the supply (inlet) tubes
into the bottles.
2. Place the priming plate on the carrier.
3. Select System > Instrument Control > Synergy H1 .
4. Click the Prime tab and select Dispenser 1.
5. Set the Volume to 5000 µL. Keep the default prime rate.
6. Click Prime to start the process. When the process is complete, carefully remove
the priming plate from the carrier and empty it.
7. Repeat the process for Dispenser 2.
Leave the water in the system overnight or until the instrument will be used again.
Purge the fluid from the system (see below) and then prime with the dispense
reagent before running an assay.
To purge the fluid from the system:
1. Place the inlet tubes in empty supply bottles or a beaker.
2. Select System > Instrument Control > Synergy H1 .
3. Click the Prime tab and select Dispenser 1.
4. Set the Volume to 2000 µL.
5. Click Purge to start the process.
6. When the purge is complete, repeat the process for Dispenser 2.
After purging the system, you may wish to run a quick Dispense protocol to visually
verify the dispense accuracy (see the next section) or the more thorough Dispense
Accuracy and Precision Tests (see
Injection System Tests starting on page 134 ).
Synergy H1 Operator's Manual
Flush/Purge Fluid Path | 61