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Troubleshooting
X
TRA
Operator’s Manual
20077/021 US
14-27
Quality of Salvaged Blood and Blood Product
The quality of the salvaged blood and resultant blood products are dependent upon good operator
techniques and knowledge of how the X
TRA
operates. Listed below in Table 14-5 are four situations
that may occur and suggested measures to overcome them.
Issue Possible Cause Possible Action
Low Hematocrit 1. Centrifuge bowl not completely filled.
May be caused by premature
triggering of red blood cell detector
due to gross hemolysis of blood in the
collection reservoir.
2. High hematocrit in collection reservoir.
3. Increased roller pump speed during Fill
or Wash cycle.
4. Dilution from saline prime to patient IV
line.
5. Custom protocol (operator-defined)
has Fill or Wash speeds set too high.
6. Custom protocol (operator-defined)
has Refill option DISABLED
1. Fill bowl in Manual until red cell
interface reaches the outside surface
of the Marker.
2. Observe how quickly the bowl fills and
decrease roller pump speeds; observe
fill volume and if low, decrease the
roller pump speed.
3. Reduce pump speeds for Fill or Wash
cycles.
4. Remove all prime from blue line before
processing.
5. Adjust parameters of custom protocol
to reduce Fill or Wash pump speeds.
6. Adjust parameters of custom protocol
to ENABLE Refill option.
Hemolysis 1. Use of hypo- or hypertonic IV
solutions.
2. Improper sampling techniques.
3. Hemolytic substances introduced into
surgical wound.
1. Use only isotonic normal saline.
2. Do not aspirate from the RBC bag with
a needle. This creates a vacuum,
causing a sampling artifact.
3. Do not aspirate hemolytic substances
into collection reservoir or aspirate
blood exposed to these substances.
Plasma Free Hemoglobin 1. Incomplete filling of centrifuge bowl.
2. Incomplete Wash cycle.
3. Excess plasma Hgb in collection
reservoir.
1. Fill bowl in Manual until red cell
interface reaches 1/8” (3.2 mm) from
white spacer.
2. Use minimum of one liter wash (1.5
liter in orthopedics) but more as
needed.
3. Control aspiration technique and
vacuum level to assure minimal
hemolysis.
Residual Heparin 1. Incomplete filling of centrifuge bowl.
2. Incomplete Wash cycle.
3. Excess heparin in collection reservoir
due to inappropriate ratios through
suction assembly or from other
sources.
4. Improper testing procedure.
5. Custom protocol (operator-defined)
has Fill or Wash speeds set too high.
1. Fill bowl in Manual until red cell
interface reaches 1/8” (3.2 mm) from
white spacer.
2. Use minimum of one liter wash (1.5
liter in orthopedics) but more as
needed.
3. Assure proper anticoagulant to blood
ratio; use low volume bowl to prevent
excess levels.
4. Do not use coagulation studies which
require the presence of plasma in
blood.
5. Adjust parameters of custom protocol
to reduce Fill or Wash pump speeds.
Table 14-5 Quality of Salvaged Blood and Blood Product