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Processing
5-24
20077/021 US
X
TRA
Operator’s Manual
Popt
The Popt protocol is intended to process the blood recovered from the operating field or the residual
volume of an extra-corporeal circuit. It has been designed to achieve an optimal compromise between
hematocrit, wash quality, and processing time.
Because the Popt protocol is a substantially automatic protocol where operator intervention is
significantly limited, only Automatic and 1 Touch modes shall be available when it is the active protocol
(refer to “The Operating Modes” on page 5-31).
Fill Phase
The Fill phase of the Popt protocol differs from the Pstd protocol and is very similar to the Pfat protocol
(different only for what concerns number of phases and flows), while it is the same as the Post-op
protocol. It consists of the following execution steps:
1. The centrifuge lid lock is engaged, the fill clamp is opened, and the centrifuge accelerates up to
5,600 rpm.
Note: If the bowl already contains blood and the buffy coat is detected before the centrifuge reaches
its programmed speed, the pump shall not start until 15 seconds after the centrifuge reaches its target
speed. This delay is intended to stabilize the buffy coat. The operator may manually start the pump
during this wait by touching the Play button. If the buffy coat is still detected after the delay, then the
pump is not started and the next step is skipped. Refer to “Automatic Reseparation Screen” on page
5-55.
2. Once the centrifuge reaches its set speed, the pump begins to rotate counter-clockwise, pumping
blood from the reservoir to the bowl, at the factory defined speed. Unlike the other protocols, the
fill speed for the Popt protocol cannot be modified. The pump continues to rotate filling the bowl
until the buffy coat is detected.
3. Once the buffy coat is detected by the RBC detector, the pump is paused for a matter of seconds
(the exact time is determined by the bowl size in use).
4. After the pause time has elapsed, the pump will continue at a second speed and pump an
additional volume of blood into the bowl until the buffy coat is detected a second time.
5. If processing with the X/55 bowl size, the Fill phase is concluded at this point and processing
moves on to the Wash phase. The procedure for all other bowl sizes proceeds to step 6.
6. Once the buffy coat has been detected, the pump will pause for a second time. The duration of
this pause is also determined by the bowl size being used.
7. After the second pause time has elapsed, the Fill phase has concluded and processing moves on
to the Wash phase.
Wash Phase
The Wash phase of the Popt protocol is identical to that of the Pstd, Pfat and Post-op protocols. It
consists of the following execution steps:
1. The 14 ml of blood in the line between the bowl and clamps is pumped back into the reservoir
line by keeping the fill clamp open, the other clamps closed, and slowly rotating the pump
clockwise for a short time.
◊ When the Wash phase is entered manually, this only takes place if at least 14 ml were
processed during the previous Fill phase.
Note: If processing with the X/55 bowl size, sub-phase where the pump moves a total amount of
25 ml of saline solution at a fixed flow-rate (50 ml/min.) begins at this point. At the conclusion of this
sub-phase, the flow rate assumes its programmable (and modifiable) value and the Wash phase
continues in the usual way.
2. The pump rotor is stopped, the fill clamp is closed, and the wash clamp is opened.
3. The pump begins to rotate counter-clockwise and reaches either the wash factory-set speed, or, if
the pump speed has been modified by the operator in a previous cycle, the new speed, moving
saline solution through to the bowl content.