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Processing
5-26
20077/021 US
X
TRA
Operator’s Manual
Pfat
The Pfat protocol is intended to process the blood recovered from the operating field. It has been
designed to remove fat particles, maintaining an optimal compromise between hematocrit and wash
quality.
Fill Phase
The Fill phase of the Pfat protocol is similar to that of Popt protocol detailed in section “Fill Phase” on
page 5-24.
Wash Phase
The Wash phase of the Pfat protocol is similar to that of the Pstd and Popt protocol detailed in section
“Wash Phase” on page 5-24.
Fat Removal Phase
The Fat Removal phase is performed only when processing with Pfat protocol. It consists of the
following execution steps:
1. Right after the regular Wash phase, an additional quantity of saline solution is pumped into the
bowl at a high flow rate. This process eliminates the residual of fat particles left inside the bowl.
This is the “Fat Removal – Wash” subphase.
2. The pump and centrifuge stop, the fill clamp closes, and the empty clamp opens.
3. The pump begins to rotate clockwise and a pre-fixed amount of RBCs is moved to the RBC bag.
This is the “Fat Removal – Empty” subphase.
4. The pump stops, the centrifuge restarts to reseparate the remaining RBCs in the bowl from the
saline solution.
5. The pump begins to rotate counter-clockwise. The RBCs previously collected in the RBC bag and,
if present, more RBCs from previous cycles are pumped back into the bowl until the buffy coat is
detected by the RBC sensor or the RBC bag is empty. This is the “Fat Removal – Conc” subphase.
Note: The Fat Removal - Empty and Conc subphases are performed in order to remove the excess of
saline solution inside the bowl after the “Fat Removal - Wash” subphase, thus avoiding excessive
dilution of the final collected RBCs.
Empty Phase
The Empty phase of the Pfat protocol is similar to that of Popt and Pstd detailed in section “Wash
Phase” on page 5-24.
Emergency
The Emergency function uses a special variation of the Pstd protocol intended to minimize the
operator’s interventions and the processing time in case of an emergency situation.
The Emergency function can be activated at any time from the Ready or Processing Screen
by touching the Emergency button.
Note: The Emergency function can be permanently enabled/disabled from the “Protocol Set” tab of
the Configuration Mode Screen. The Emergency button will only be available when it is enabled. For
instructions on configuring the X
TRA
, refer to
Chapter 8: Configuring Xtra
.
When the Emergency button is touched, the Emergency Activation Screen (see Figure 5-28) is
displayed, requiring confirmation before the function is activated.