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Preoperative Sequestration (PPP and PRP)
X
TRA
Operator’s Manual
20077/021 US
10-21
The Preoperative Sequestration Factory Protocols
The following sections describe only the preoperative sequestration protocols which ship with the X
TRA
system: PPP, PRP1, and PRP2. For an overview of what protocols are and for specific description of the
intraoperative and postoperative blood recovery protocols, refer to “Factory Protocols” on page 5-22.
For instructions on running a case using these protocols, refer to the section “Running a Preoperative
Sequestration Case” on page 10-9.
Overview of the Preoperative Sequestration Protocols
The preoperative sequestration protocols (PPP, PRP1, and PRP2) will separate whole blood previously
collected in a bag into its components. The plasma and platelets will be sequestered in the collection
bag(s), and the remaining blood components will be collected in the RBC bag.
The
X
TRA
ships with three sequestration protocols:
• PPP: For the collection of platelet poor plasma only in the PPP bag,
• PRP1: For the collection of both plasma and platelets in a single bag, and
• PRP2: For the collection of platelet poor plasma in the PPP bag and then the platelet rich plasma
in a separate PRP bag.
For a detailed account of each phase of the sequestration process, refer to “Overview of Preoperative
Sequestration” on page 10-2.
While processing with the SEQ protocols, the X
TRA
will be set up with a 3-branched collection line
connected to the bowl outlet (see Figure 10-13):
• The waste line will remain clamped during all SEQ protocols.
• The PPP line will be open at the start of all protocols. During the PRP2 protocol, the PPP branch
will be clamped by the operator and the PRP branch opened so that PRP may be collected in a
separate bag.
• The PRP bag will be clamped at the beginning of each protocol. During the PRP2 protocol this line
will be opened by the operator during the Spill PRP phase so that PRP may be collected. During
the PPP and PRP1 protocols, this line may be used as a second bag if the first one becomes full.
Figure 10-13 Disposable Setup for Sequestration Protocols