Beckman Coulter Navios, Instructions For Use Manual

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773232AH
4-34
Specifications
Performance Characteristics And Specifications
Scatter Resolution
Scatter resolution on the Navios Flow Cytometer was measured using 0.404 μ m particles from
Thermo Scientific. The noise was set to the bottom of the scale, using FS HV = 700, Gain at 200,
Discriminator at 47 and setting the Forward Scatter detector to the Wide2 setting. The Navios was
able to clearly show baseline resolution between the noise and the 0.404
μ m diameter particles.
Forward Scatter
The HPCV of the integral signal intensity values using Flow-Check Pro fluorospheres is <2% from the
blue laser.
Fluorescence
The HPCV of the integral signal intensity values using Flow-Check Pro fluorospheres is <2% for FL1-
FL4 and <2.5% for FL5 from the blue laser, <3.0% for FL6-FL8 from the red laser and <4.0% for FL9-
FL10 from the violet laser.
Sensitivity
Fluorescence
Three measurements of multi-level fluorescence sensitivity particles were taken on 3 Navios Flow
Cytometers. Acquisition was conducted on 10,000 bead stop count at the medium flow rate. For the
FITC and PE measurements, 8 peak Spherotech Rainbow Calibration Particles (RCP-30-5A) were
used. For the PeCY5, and APC, Spherotech Ultra Rainbow Calibration Particles (URCP-38-2K) were
used. The following values represent the average of the measurements taken.
•
<112 MESF for FITC
•
<78 MESF for PE
•
<15 MESF for PC5
•
<75 MESF for APC
Laser Characteristics
Day-To-Day
The mean channel value of the integral signal intensity of alignment verification fluorospheres
from the standard PMT’s off the blue, red and violet lasers does not vary more than ±5% from any
integral signal channel number obtained over a period of 8 days.
Within Day
The mean channel value of the integral signal intensity of alignment verification fluorospheres
from the standard PMT’s off the blue red, and violet lasers does not vary more than ±5% from any
integral signal channel number obtained within a period of 24 hours.