Beckman Coulter Navios, Instructions For Use Manual

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773232AH
3-9
Operation Principles
Signal Processing
3
Time-Of-Flight Signal
Time-of-Flight is the transit time of a cell or particle to traverse the laser beam. The pulse width of
a peak pulse is measured and can be assigned to any peak signal. An application for Time-of-Flight
is doublet discrimination.
Figure 3.9
Time-Of-Flight Pulses
Amplification
Some voltage pulses must be amplified so that the characteristics of the cells can be measured. The
system lets you:
•
Increase the gain to linearly amplify the integral, peak and Time-of-Flight signals.
•
Logarithmically transform the linear intregral and peak signal data.
A logarithmic transformation accentuates the differences between the smaller pulses and reduces
the differences between the larger pulses.
1.
Pulse height/shape
2.
Time
b
c
2C 4C 2C + 2C = 4C