Beckman Coulter Navios, Instructions For Use Manual

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773232AH
3-5
Operation Principles
Light Collection, Separation And Measurement
3
Figure 3.3
Side Scatter Collection
Fluorescent Light
Band pass and Long pass filters are used to transmit color bands. The color bands are designed to
measure fluorescence light from the fluorochromes such as FITC, PE, ECD, PC5, PC5.5, PC7, APC, APC
AlexaFluor700, APC AlexaFluor750, Pacific Blue and Krome Orange (with PMT and violet laser
upgrades installed) that are excited by illumination from the lasers. Dichroic filters are used to
reflect colors. Positions of the dichroic filters have been efficiently designed to reduce the number
of optical surfaces fluorescence light must pass to reach the photo sensors. Their locations relative
to the optical axis have also been optimized for light to pass symmetrically through each filter. You
can individually interchange the optical filters. There is no need to realign the optical system when
the filters are changed.
Figure 3.4
Two Laser, 6 Color Filter Block Configuration
1.
Violet laser beam
2.
Blue laser beam
3.
Red laser beam
4.
Side scatter collected through fiber optic cable
mounted on the right side of flow cell
b
c
d
e
525 BP
40
575 BP
30
695 BP
30
620 BP
30
755 LP
660 BP
20
550 DC SP
595 DC SP 730 DC SP
655 DC SP 710 DC SP
620 BP 30
755 LP 660 BP 20
550 DC SP
655 DC SP 710 DC SP
595 DC SP
730 DC SP
525 BP 40
575 BP 30 695 BP 30/
675 BP 20