Beckman Coulter Navios, Instructions For Use Manual, Page: 69 / 586

773232AH
3-15
Operation Principles
Plot Display
3
Histogram Statistics
Linear Region Statistics
For linear signals, statistics for histogram regions are calculated as follows:
Number = Number of cells in the region
Mode = Intensity containing the largest number of cells within the region
Total is expressed as a percent (%).
Where d = Standard deviation
CV = Coefficient of Variation (expressed in % CV)
M
H
= High region marker channel
M
L
= Low region marker channel
C
n
= Raw digital channel number
C
ch
= Channel count
N = Integral of all counts between & including marker channels
= Mean channel number (rounded to nearest whole channel)
For Mean and SD, the summations are performed over all the channels that lie within the region.
Median intensity = the smallest intensity such that sum (intensity) 
Sum (intensity) = sum of events from the lower edge of the region to the intensity
Where FWHM=the Full Width Half Max value of a Normal or Gaussian peak.
Log Region Statistics
The method of geometric calculations of Means depends on your Advanced Statistics Configuration .
total
Number of events in region
Total number of events in the gated display
-----------------------------------------------------------------------------------------------------------
100
×
=
total
Number of cells in region
Total number of cells in the file
------------------------------------------------------------------------------
100
×
=
Mean median intensity 0.5
sum median intensity
( )
area
2
-----------
–
count in median intensity
--------------------------------------------------------------------------------
– + =
area
2
-----------
Half Peak CV
FWHM
2.354
--------------------
=