Beckman Coulter Navios, Instructions For Use Manual

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773232AH
4-9
Specifications
Software Specifications
4
The initial protocol in each application, which you use to adjust or confirm correct PMT High
Voltage and parameter gain settings, utilizes Flow-Set Pro fluorospheres.
If you wish to use a negative control sample use the “AS *_STAND.Pro” protocol in the above panels.
You must modify the protocol for the number of colors you are analyzing.
Each AutoSetup II application requires a user defined base protocol with the desired parameter
names to generate the application. The Application Definition Wizard creates a unique base
protocol with the same name as the application.
The guidelines below show approximate region widths generated by the Application Definition
Wizard.
Flow-Set Pro region widths generated by the Application Definition Wizard may be
edited.
If different regions widths are desired, open the appropriate *_Stand protocol onto the workspace
and edit the regions. Save the protocol. Subsequent scheduling of the application uses the edited
regions.
•
Forward Scatter (linear): ±20
•
Side Scatter (linear): ±50
•
Fluorescence Parameters (logarithmic);
•
1st decade - beginning or low end: ±0.1
•
1st decade - high end: ±0.3
•
2nd decade - beginning or low end: ±1.0
•
2nd decade - high end: ±3.0
•
3rd decade - beginning or low end: ±10
•
3rd decade - high end: ±30
•
4th decade - beginning or low end: ±100
•
4th decade - high end: ±300
Default Generic Panels and Protocols
The following generic protocols are included with Navios software.
Application
Name Flow-Set Pro Protocol Compensation
Protocols Verification Protocol Settings Protocol Base Protocol
AS 6C 2L AS 6C 2L_STAND.Pro AS 6C 2L_FL01.Pro
AS 6C 2L_FL02.Pro
AS 6C 2L_FL03.Pro
AS 6C 2L_FL04.Pro
AS 6C 2L_FL05.Pro
AS 6C 2L_FL06.Pro
AS 6C 2L_VERIFY.Pro AS 6C 2L
Settings.Pro AS
6C2L_APP.Pro
Panel Name Protocol