Beckman Coulter Navios, Instructions For Use Manual, Page: 543 / 586

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Glossary-3
Glossary
Fixed code length
- A specific length of sample ID code, (usually enabled when all sample IDs are the
same length) to make sure that only one length sample ID is accepted.
Flow cell
- A device through which particles pass, in a stream of fluid, one at a time, through a laser
beam.
Flow cytometry
- A process for measuring the characteristics of cells or other biological particles as
they pass through a measuring apparatus in a fluid stream.
Flow Cytometry Standard (FCS)
- The flow cytometry data file standard that provides the
specifications needed to completely describe flow cytometry data sets within the confines of the file
containing the data.
FlowPAGE
- A template for creating a report. It can include: data plots, statistics, and user attached
text and/or graphics.
Fluorescent light
- The emission of electromagnetic radiation that occurs when the emitting body
absorbs radiation from some other source. For example, when a fluorescent dye is excited (absorbs
radiation), it emits fluorescent light at a wavelength that is different from the wavelength of the
light that excited it.
Fluorescent light (FL1, FL2, FL3, FL4, FL5, FL6, FL7, FL8, FL9 and FL10) sensors
(PMTs) - Collect the
fluorescent light and generate voltage pulse signals. The 1 refers to the first fluorescence sensor; 2
the second; and so forth.
Forward scatter (FS)
- The laser light scattered at narrow angles to the axis of the laser beam. The
amount of forward scatter is proportional to the size of the cell that scattered the laser light.
Forward scatter (FS) sensor
- Collects the forward scatter and generates voltage pulse signals.
Gain
- The amount of amplification applied to a signal. In linear amplification, all of a sensor's
signals are increased by the same amount. Contrast with logarithmic amplification.
Gating
- The use of criteria that must be met before an event is included in a histogram.
GB
- The abbreviation for gigabyte.
High voltage
- Can be adjusted to change the sensitivity of a fluorescent light sensor.
Histogram
- A graph showing the relative number and distribution of events.
HPCV - Half peak coefficient of variation
Hydrodynamic focusing
- A process that focuses the sample stream through the flow cell. It ensures
that cells move through the laser beam one at a time, along the same path.
Indicators
- See Controls and Indicators.
Integral signal
- A voltage pulse with height and area proportional to the total amount of fluorescent
material in a cell.
Intercharacter gap
- The space between two characters in a bar-code symbol. Refer to discrete code.
Not in all bar-code types.