Beckman Coulter Navios, Instructions For Use Manual

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773232AH
4-16
Specifications
Software Specifications
$PnB Number of bits allocated to store data for parameter n: 32 = 20 bit data per
parameter plus 12 bits of padding.
$PnE Lin/Log parameter calibration. This value is always “0,0” and it means linear.
$PnN Name of parameter n, for example: Manufacturer assigned name, that is: SS-A,
FL2-H, FL3-W.
$PnR Channel resolution of parameter n: 1048576 = 20 bit parameters.
$SPILLOVER Compensation matrix. The matrix is size is specified in the first value of the
keyword. The following values indicate the index of each of the parameters listed
in the matrix.
The actual values in the matrix (Sij) are floating point numbers that indicate the
ratio of the amount of signal in the “j” channel to the amount of signal in the “i”
channel for particles carrying only the “i” parameter dye.
Only parameters associated with fluorescence detectors are present in this
compensation matrix.
NOTE
This keyword is not part of the FCS 3.0 standard. It has been included
based on a pre release version of the FCS 3.1; its implementation does not
match the released version of the FCS 3.1 standard.
$TOT Total number of events acquired.
@BARCODE Barcode read from the sample loader or “NoRead” if nothing is read.
@BLUELASERPOWER_END Blue laser power at end of acquisition.
@BLUELASERPOWER_START Blue laser power at start of acquisition.
@BLUELASERSHUTTER Blue laser shutter status: “Open” or “Closed”.
@BLUETARGETPOWER Blue laser target power.
@CHECKSUM FCS data checksum.
@CAROUSEL Carousel number read from the sample loader.
@CRS20BITFORMAT Data stored in CRS 20bit format. This value is always “TRUE”
@CYTOMETERID The unique ID given to the flow cytometer by the user, for example: the flow
cytometer serial number.
@PnADDRESS Multiplexer address of parameter n.
@REDLASERPOWER_END Red laser power at end of acquisition.
@REDLASERPOWER_START Red laser power at start of acquisition.
@REDLASERSHUTTER Red laser shutter status: “Open” or “Closed”.
@REDTARGETPOWER Red laser target power.
@SAMPLEID1 User defined string.
@SAMPLEID2 User defined string.
@SAMPLEID3 User defined string.
@SAMPLEID4 User defined string.
@TESTFILE Protocol file name.
Table 4.2
FCS 3.0 Keyword Information
FCS 3.0 Keyword Key Value