NEW BRUNSWICK SCIENTIFIC BioFlo 415, Manual To Operations

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BioFlo 415
y
M1360-0050 User’s Guide
3.7 DO Control
DO is controlled in the range of 0-200% (
±
1%). It is sensed by the DO electrode and
control is maintained by the P & I controller by changing the speed of agitation, the
thermal mass flow controller-regulated flow rate (if your system is so equipped),
nutrient addition, and/or the percentage of oxygen in aeration. The DO probe is a
polarographic probe.
Default P & I (proportional & integral) values are preset at the factory.
We strongly
recommend that you maintain the factory-set parameters.
3.8 High Foam Control
Foam can be controlled during batch fermentation by a level probe located in the
headplate. The controller operates the antifoam-assigned pump that adds chemical
defoamer into the vessel as needed.
3.9 Exhaust System
The exhaust gases pass into the exhaust condenser
(see Figure 5)
where moisture is
removed; any residual moisture is heated past the dewpoint via the heating pad,
allowing it to pass through the exhaust filter with a reduced potential for clogging.
3.10 Sampling/Harvesting System
The sampler assembly consists of the sampler tube with a sampler needle, three tri-
clamps and a sampler valve. This assembly facilitates the aseptic removal of the
sample, or harvesting the entire contents of the vessel, to an optional sample/harvest
vial or bottle.