NEW BRUNSWICK SCIENTIFIC BioFlo 415, Manual To Operations

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BioFlo 415
y
M1360-0050 User’s Guide
7.3.2 DO Probe Preparation
To ensure stable output, the probe should be sent through two or three
sterilization cycles prior to use. The probe will be operable after the second
cycle, but it will be more stable with additional sterilizations.
Default P & I (proportional & integral) gains are preset at the factory.
It is
strongly recommended that you maintain the factory-set parameters.
Even if the power fails during a run, the pre-set P & I values are stored in
memory and should still be in effect when the power is restored.
Nevertheless, it is always prudent to check these values at the beginning of a
run, especially if the fermentor has not been used for a while or if other people
have access to the unit.
#
NOTE:
It is recommended that you use the factory-set P & I values. Do not
attempt to change the settings unless you are very experienced with P & I
control.
7.3.3 DO Probe Installation
Install the DO probe into the vessel headplate assembly
(see Figures
3a & 3b, and Figure 28 on the following page)
, ensuring that there is no
interference inside the vessel and taking care to never strike or bump the tip of
the probe. To install the probe without an adapter:
1.
Apply a light coat of glycerol to the sides of the probe.
2. Carefully insert the probe into the port, gently rotating it as you glide it into
place.
3. When it is fully seated, finger tighten the lock nut.
If you are using an adapter for a 19mm port:
1.
Screw the adapter into the port until it is finger-tight.
2. Carefully insert the probe into the adapter.
3. Finger tighten enough to compress the O-ring to ensure a tight seal.