NEW BRUNSWICK SCIENTIFIC BioFlo 415, Manual To Operations

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BioFlo 415
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M1360-0050 User’s Guide
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After becoming familiar with the components and operation of each of the systems,
subsystems, and options that are included on your BioFlo 415, you are ready to set up the
fermentor/bioreactor for a run.
#
NOTE
:
When preparing the fermentor/bioreactor for a run, always make sure
that all hardware is set up to control the process prior to entering any
setpoints or activating the system at the controller.
Before initiating the fermentation run
, consult the following checklist to be sure all is
ready:
† Sterilization has been completed (
see Section 8
)
† The DO probe has been recalibrated (
see Section 7.3
).
† The pH and DO control loops have been set to
AUTO
mode.
† The desired cascades have been enabled (
see Section 11
).
† The pumps have been set to run and the liquid addition system is in place
(
see Sections 16.1 - 16.2
)
† Inoculate the vessel (
see Section 16.3
).
† If you are using NBS supervisory software, start
BioCommand
(
see Section 16.4
).
During the run
:
† To conduct sampling, see Section 16.5.
† To review the various phases of fermentation, see Section 16.7.
† To review varieties of fermenation runs, consult Sections 16.8 through 16.10.
† To harvest the product of your run, see Section 16.6.
At the end of the run
:
† To shut down at the end of the run, follow the instructions in Section 16.11.
†
Clean the vessel according to Section 17.1.
16.1 Load Pump Tubing
The three standard pumps are located on the front of the control cabinet (
see Figure 42,
repeated on the following page for easy reference):