NEW BRUNSWICK SCIENTIFIC BioFlo 415, Manual To Operations

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104
BioFlo 415
y
M1360-0050 User’s Guide
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NOTE:
In order to increase the flow rate of the sample, temporarily disconnect the
hose from the
MAIN EXHAUST OUT
connection on the side of the cabinet.
16.6 Harvesting
At the end of a batch, to harvest the entire culture:
1.
Aseptically prepare an autoclaved harvest bottle large enough to hold the entire
contents of the vessel. The bottle cap should be equipped with a sterile filter on
one penetration. There should also be sufficient silicone tubing on the other
penetration to reach from the bottle, placed on the floor below the benchtop, to the
sample assembly outlet. Secure both the tubing and the filter with plastic ties.
2.
Aseptically install the harvest tubing on the sampler outlet. Secure the harvest
tubing on the sampler outlet with a plastic tie.
#
NOTE:
In order to increase the flow rate of the harvest, temporarily disconnect
the hose from the
MAIN EXHAUST OUT
connection on the side of the cabinet.
3. Following the sampling instructions provided in Section 16.5 above (
Sampling
Procedure
) to harvest the entire contents of the vessel. Note that a small residue
will probably remain in the vessel.
Alternatively, you can use a feed pump to harvest the vessel contents.
When the
vessel is set up on the control cabinet, adjust the level probe’s tip to the level at which
you want harvesting to stop (i.e., below the current liquid level):
1. Assign a feed pump as
Lvl2 Wet
, to pump liquid out of the vessel.
2. Aseptically connect the feed pump’s tubing to the harvest port.
3. Turn the pump ON . Since the liquid is in contact with the probe, the circuit will
close, and the pump will begin pumping liquid out of the vessel.
4. When the liquid drops below the probe tip, the pump will stop.
See also Section 10.2,
Pump Assignment
. If you assign the pump to None instead of
Lvl2 Wet , it will harvest as much as possible.
16.7 Fermentation Phases
In a typical fermentation run, you can expect to see four characteristic phases: Lag,
Exponential Growth, Steady State, and Decline.