NEW BRUNSWICK SCIENTIFIC BioFlo 415, Manual To Operations

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BioFlo 415
y
M1360-0050 User’s Guide
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CAUTION!
Never clean the vessel or its components or the control cabinet with
abrasive chemicals or materials.
17.1 Cleaning the Vessel
After every fermentation run, we strongly recommend that you clean the vessel
according to the following procedure:
1.
Rinse the vessel with tap water as soon as the run is completed, then drain it.
#
NOTE
:
If applicable, be sure to follow the bio-safety regulations regarding the
release of cultured microorganisms into the environment.
2. Fill the vessel with tap water again, up to or near the surface of the headplate.
3.
Keep Agitation at 200-300 rpm without aeration for about one hour at a
temperature setpoint of 30 – 35°
C.
4.
Drain the water while the Air Inlet valve is open; set Gas flow rate at 5-10 L/min,
then close it.
5.
Fill the vessel with tap water again, up to or near the headplate, and leave the
Agitation on at 200 rpm for an hour or overnight.
6. Drain the water before the next run.
17.1.1 List of Wetted Parts
For further reference in your choice of cleaning detergents, Table 11 (
on the
following page
) provides a list of wetted parts in the vessel assembly and the
materials they are made of: